Measuring DNA Damage and Repair in Mouse Splenocytes

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Supplied as 100 µL purified antibody (1 mg/mL). Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage. A gamma-H2AX kit and antibody allows the assessment of DNA damage and DNA repair for ELISA based assays, immunohistochemistry or flow cytometry. In the detection of genomic damage, gamma-H2AX is especially important in research involving cancer treatment and therapy, and environmental biomonitoring. Exclusively from The Antibody Resource, you can buy this Superstarter kit, which is a collection of trial sizes of these Superstar phospho-Histone H2A.x antibodies (highly rated and independently tested by us), to test using the specific experimental conditions which your research dictates, so that you can identify which will be the most appropriate for your research project.

In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. Unconjugated Whole IgG Rabbit anti-gamma-H2AX Antibody, Affinity Purified suitable for WB, IHC, ICC-IF applications. Visit Bethyl.com for all your antibody needs. Bethyl is now a part of Fortis Life Sciences. Balajee, A. S. & Geard, C. R. Replication protein A and gamma-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks. Exp Cell Res 300, 320–334 (2004). CAS Article H2AX is a reported alias name for the human gene H2AFX, or 'H2A histone family member X'. The 143-amino acid protein is a member of the Histone H2A family.

The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair 5 Mar 2021 Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4).

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Success from the first experiment – confirmed specificity through extensive validation. Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4).

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. 2016-03-31 Monoclonal Antibody for studying H2AX (Ser139) phosphate in the Chromatin Regulation / Epigenetics research area. I use MCF-7 cell, treated with 0.5uM Doxorubicin 24 hours, did WB, and used two different gamma H2AX antibodies to detect ( #2577 in Cell signaling, rabbits and #05-636 in Millipore, mouse) I Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites.
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Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications.

Applications, ICC-IF, IHC, WB. Target, gamma-H2AX. Type, Cell Biology Antibody.
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The H2AX variant represents between 2-25% of total H2A (1-3). gamma-H2AX Antibody, A300-081A.